全自动化学发光免疫分析仪基本原理简介

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                    <section><section powered-by="gulangu"><section><section><p>基本原理</p></section> <p><img src="image/20201019/e9a2b28370ba261055ff28ae09f18efd_1.png" /></p> <section></section></section></section><section powered-by="gulangu"><section><section><p>&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; 反应体系中的某些物质的分子（反应物、产物、中间体或荧光物质）吸收了反应所释放的能量而由基态跃迁至激发态，然后再从激发态返回基态，同时将能量以光辐射的形式释放出来，产生化学发光。</p><p>&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; 将化学发光反应应用于分析化学，根据某一时刻的发光强度或反应的发光总量来确定体系的相应组分含量的分析方法叫化学发光分析法。</p><p>&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; 化学发光的反应必须具备两个条件：</p><p>&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; 一是该反应能释放出一定的能量，且释放出的能量可以被某种反应产物或中间体所吸收，使之处于激发态。</p><p>&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; 二是这种激发态产物应具有一定的化学发光量子产率，或者可以将其能量有效地转移给某种荧光物质，产生光辐射。</p><p>&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; 因此，并不是任何化学反应都能产生化学发光反应，因此，如果测量条件适当，化学发光分析会有足够的选择性。</p><p>&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; 化学发光免疫分析仪采用光电倍增管（PMT）将单光子检测并传输至放大器，并加高压电流放大，放大器将模拟电流转换化为数字电流，数字电流将发光信号由RS232数据线传输给电脑并加以计算，得出临床结果。</p></section></section></section><section powered-by="gulangu"><section><section><p>化学发光的分类</p></section> <p><img src="image/20201019/e9a2b28370ba261055ff28ae09f18efd_1.png" /></p> <section></section></section></section><section powered-by="gulangu"><section><section><p>&nbsp; &nbsp; &nbsp; &nbsp; &nbsp;化学发光反应参与的免疫测定分为二种类型。</p><p>&nbsp; &nbsp; &nbsp; &nbsp; &nbsp;一种是以发光剂作为酶免疫测定的底物，通过发光反应增强测定的敏感性。如化学发光酶免疫测定（CLEIA，与酶标ELISA法类似，即最后一步酶反应所用底物为发光剂）。</p><p>&nbsp; &nbsp; &nbsp; &nbsp; &nbsp;另一种是以发光剂作为抗体或抗原的标记物，直接通过发光反应检测标本中抗原或抗体的含量。如化学发光标记免疫测定亦称化学发光免疫测定（CLIA）、电化学发光免疫测定（ECLI）。</p></section></section></section><section powered-by="gulangu"><section><section><p><br  /></p></section></section></section><section powered-by="gulangu"><section><section> <section powered-by="gulangu"><section><section><p>欢</p></section></section></section> </section><section> <section powered-by="gulangu"><section><section><p>迎</p></section></section></section> </section><section> <section powered-by="gulangu"><section><section><p>关</p></section></section></section> </section><section> <section powered-by="gulangu"><section><section><p>注</p></section></section></section> </section></section></section><section powered-by="gulangu"><section><section><p><br  /></p></section></section></section><section powered-by="gulangu"><section><section> <section powered-by="gulangu"><section><section><p><img src="image/20201019/d6f80f0ca5b7d42ad045e6b46d5ac326_3.jpg" /></p></section></section></section> </section><section> <section powered-by="gulangu"><section><section> </section></section></section> </section><section> <section powered-by="gulangu"><section><section><p>关注我们</p><p>一起涨姿势！</p></section></section></section> </section></section></section></section>