干货丨全套常见核酸提取仪、PCR仪品牌、型号、通量

admin · 发表于 2020-10-14 12:18:41

                  <section data-role="paragraph" data-color="rgb(182, 228, 253)" data-custom="rgb(182, 228, 253)"><section><section><section><section powered-by="gulangu"><section><section><section><section powered-by="gulangu"><section><section><section><section powered-by="gulangu"><section><section><section><section powered-by="gulangu"><section><section><section><section><p><p><img src="image/20201014/86b79d371e9862b01189f0252a03b2cd_1.png" /></p></p></section><section><section><span><strong></strong></span></section><p><span>医疗器械媒体报道先锋</span></p><p><span>分享专业医疗器械知识</span></p></section><section><section><section><span>关注</span></section></section></section></section></section></section></section></section></section></section></section></section></section></section></section></section></section></section></section></section></section></section></section><section><section><section><section><section><section><br  /></section><p><span>资料来源：IVD销售实战课堂（ID：IVD0626）</span></p><section data-support="96编辑器" data-style-id="5462"><section><section data-width="65%"><br  /></section></section></section><p><strong><span>聚合酶链式反应（PCR）</span></strong>，<span>是一种用于放大扩增特定的DNA片段的分子生物学技术，它可看作是生物体外的特殊DNA复制，PCR的最大特点是能将微量的DNA大幅增加。因此，无论是化石中的古生物、历史人物的残骸，还是几十年前凶杀案中凶手所遗留的毛发、皮肤或血液，只要能分离出一丁点的DNA，就能用PCR加以放大，进行比对。这也是“微量证据”的威力之所在。由1983年美国Mullis首先提出设想，1985年由其发明了聚合酶链反应，即简易DNA扩增法，意味着PCR技术的真正诞生。到如今2013年，PCR已发展到第三代技术。1976年，中国科学家钱嘉韵，发现了稳定的Taq DNA聚合酶，为PCR技术发展也做出了基础性贡献。</span></p><p><br  /></p><p><span>Khorana (1971)等最早提出核酸体外扩增的设想：“经DNA变性，与合适的引物杂交，用DNA聚合酶延伸引物，并不断重复该过程便可合成tRNA基因。”</span></p><p><span><br  /></span></p><p><span>但由于当时基因序列分析方法尚未成熟，热稳定DNA聚合酶尚未报道以及引物合成的困难，这种想法似乎没有实际意义。加上分子克隆技术的出现提供了一种克隆和扩增基因的途径，所以Khorana的设想被人们遗忘了。</span></p><p><span><br  /></span></p><p><span>1985年，Kary Mullis在Cetus公司工作期间，发明了PCR。Mullis要合成DNA引物来进行测序工作，却常为没有足够多的模板DNA而烦恼。</span></p><p><span><br  /></span></p><p><span>1983年4月的一个星期五晚上，他开车去乡下别墅的路上,猛然闪现出“多聚酶链式反应”的想法。</span></p><p><span><br  /></span></p><p><span>1983年12月，Mullis用同位素标记法看到了10个循环后的49 bp长度的第一个PCR片段；</span></p><p><span><br  /></span></p><p><span>1985年10月25日申请了PCR的专利，1987年7月28日批准（专利号4,683,202 ），Mullis是第一发明人；</span></p><p><span><br  /></span></p><p><span>1985年12月20日在Science杂志上发表了第一篇PCR的学术论文，Mullis是共同作者；</span></p><p><span><br  /></span></p><p><span>1986年5月，Mullis在冷泉港实验室做专题报告，全世界从此开始学习PCR的方法。</span></p><p><br  /></p><p><br  /></p><p><span><strong>◆</strong><strong>  ◆  ◆</strong></span><br  /></p><section><p><span>常见核酸提取仪品牌、型号大全</span></p><p><br  /></p><p><p><img src="image/20201014/6e94972175d9cc61d1ecb3fb930a47db_2.png" /></p></p><p><p><img src="image/20201014/e4e7ad5363bc2c51e18e03717ddb6c08_3.png" /></p></p><p><p><img src="image/20201014/1d1ca3ad4676941d0595f8b59f2ab5ab_4.png" /></p></p><p><p><img src="image/20201014/2a1d56aa1c16fc74e87f227c9419d47b_5.png" /></p></p><p><p><img src="image/20201014/1c20cabdf7d681669c566fdc96669ddf_6.png" /></p></p><p><p><img src="image/20201014/13a87fef9e88c9789ee178cdca4736f5_7.png" /></p></p><p><p><img src="image/20201014/bbcee566f580d78ac58b3e520347f352_8.png" /></p></p><p><p><img src="image/20201014/dff30d6e993776e497b1515be3da92e2_9.png" /></p></p><p><p><img src="image/20201014/4a8ccc7d4a257957e71dcf1c9f7f050b_10.png" /></p></p><p><p><img src="image/20201014/f6b359f3b9fe6b975d90cf1087092245_11.png" /></p></p><p><p><img src="image/20201014/6ab18b7e2e26b21866ab65b669eb8185_12.png" /></p></p><p><br  /></p></section><p><span><strong>◆</strong><strong>  ◆  ◆</strong></span><br  /></p><section><p><span>老牌常见PCR仪品牌、型号大全</span></p></section><p><br  /></p><p><p><img src="image/20201014/2e471a511eac2caabb3a699042ca5495_13.png" /></p></p><p><p><img src="image/20201014/8a1f2dc64f9635e621420ac43919ff8f_14.png" /></p></p><p><p><img src="image/20201014/7a365d42fda9c5add0563344033f2ab9_15.png" /></p></p><p><p><img src="image/20201014/73060bb9fb530152f11da81e8cd305e0_16.png" /></p></p><p mpa-paragraph-type="body"><br  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