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<blockquote><p><span>让天下没有难找的医疗器械服务信息!</span></p><p><strong><span>“医疗器械助手”</span></strong><span> 让一切触手可及!</span></p><p><p><img src="image/20201014/ac7c4c86bff3f0660e634292dc511095_1.jpg" /></p></p><p><span>点击图片</span><span><span></span><p><img src="image/20201014/c407fe90207c2622cc8e1dff32d28341_2.jpg" /></p><p><img src="image/20201014/c407fe90207c2622cc8e1dff32d28341_2.jpg" /></p><p><img src="image/20201014/c407fe90207c2622cc8e1dff32d28341_2.jpg" /></p></span></p><p><br /></p></blockquote><p><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">日前,科学家杂志(The Scientist)评选出了2017年度的十大医疗技术发明。<br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><strong>1. IsoCode芯片</strong></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box" center="center"><p><img src="image/20201014/8e4caa3416db521ceda28119d1ce89d1_5.jpg" /></p></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">这是一种新的单细胞技术,其能让研究人员能够根据细胞分泌的蛋白质对细胞进行特征分析,可以同时分析多达42种不同的细胞因子,趋化因子和其他分子。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">IsoCode芯片由Isoplexis公司于今年2月份推出,它包含数千个只能容纳单细胞的微型腔室,在每个微室内,15个分离空间的槽内包含多达三种针对特定分泌蛋白的不同抗体,结合后,抗体发出三种颜色的荧光,使研究人员能够区分蛋白质。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">目前,IsoCode芯片的潜在应用之一是分析CAR-T细胞,IsoPlexis公司首席执行官Sean Mackay表示:“IsoCode芯片能够同时分析数千个单独的T细胞或免疫细胞,更重要的是,每个免疫细胞能够获得30到45个分泌蛋白,而以往的技术要么大量地测量细胞,要么丢失颗粒度,要么只能看到每个细胞的少量分泌蛋白质,因此,IsoCode芯片是真正的创新”。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">科学家杂志的评审专家也评论到:“IsoCode单细胞技术,易于使用,有可能影响癌症研究的生物标志物发现和患者监测。”</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><strong>2.雅培手持血液仪i-STAT Alinity</strong></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box" center="center"><p><img src="image/20201014/966ef97d1be2313e85850f798968866f_6.jpg" /></p></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">雅培公司最新版本的手持式血液检测设备i-STAT Alinity具备了很多创新的功能,它的体积只有80年代的手机大小(<span>很适合医护人员随身携带</span>),却拥有三十年前难以想象的技术,i-STAT Alinity可以对几滴血样进行无数次测试,并在几分钟内将包括葡萄糖水平和血细胞比容的结果传送给医生,使得即时检测变得更加快速、便捷。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">雅培护理诊断业务研发总监纳Narendra Soman表示:“i-STAT的一项改进是大型彩色触摸屏,同时,如果患者的指标超出正常范围,则会向用户发出声音和视觉提示,此外,我们为测试结果还增加了更多功能,一旦获得血液检测结果,就可以从仪器传输到病人的病历”。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">一年前,i-STAT Alinity正式上市,目前已在大约四十多个国家有售(<span>不包括美国</span>),售价在7000到12500美元之间。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><strong>3. 实验室培育类固醇激素:QGel</strong></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box" center="center"><p><img src="image/20201014/a15227e06de1b7d49ec817f878e507ff_7.jpg" /></p></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">这个产品利用动物源性细胞外基质(ECM)技术,从患者的癌细胞种植类固醇,并用于制作个性化治疗,目前已在抗肿瘤研究中得以使用,比如,诺华就利用QGel来培育用于药物筛选用的癌细胞,和来自患者自身的细胞。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">QGel公司的联合创始人Colin Sanctuary表示,如果使用QGel而不是动物来源的ECMs来培育,那么在临床治疗中的应用将能够达到全球范围的规模,并对受影响的人群将产生重大影响。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">QGel的成本大约在4,000美元至5,000美元之间。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><strong>4.Blaze系统</strong></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box" center="center"><p><img src="image/20201014/e32a56e7ef6bb479f87d00084916d26b_8.jpg" /></p></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">Intabio公司的Blaze系统用于检测和鉴定蛋白质异构体,可以为制药公司在实验室的准备工作中节省大量的时间,”通常需要一个月的蛋白质分析,Blaze一天内就能完成。“Intabio公司首席执行官Lena吴表示。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">Blaze系统将检测、定量、鉴定整合到一个微流体系统中,该系统能够在检测后立即发送蛋白质用于质谱分析,避免了单独为质谱分析准备材料的繁琐过程,以此加快了检测的速度,”它彻底改变了能获得产品质量关键信息的途径。“Lena吴说道。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">Blaze系统将在几个月后正式上市,定价尚未确定,不过有专家预估该设备价格在7万美元至20万美元之间。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><strong>5、SR-X超敏感生物标志物检测系统</strong></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box" center="center"><p><img src="image/20201014/4e0d0a428fa77178f7bb01cf8d0b2708_9.jpg" /></p></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">今年8月,Quanterix公司正式推出SR-X系统,该生物标志物检测系统可以提供超过80种不同的分析方法来测试样品,通常是血液或血清,不过,也能通过分析脑脊液或单细胞裂解液来检测细胞因子,神经变性或神经炎症等其他标志物。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">Quanterix公司产品战略总监Jeremy Lambert表示:SR-X的核心技术Simoa使用更多的磁珠相作用于它们的目标蛋白质,每个珠只捕获一个蛋白质,然后将那些携有蛋白质的颗粒沉淀、洗涤,再与抗体检测器结合,并流过可容纳单一颗粒的200,000个微室的阵列,在那里,抗体检测器与荧光报道分子相互作用,SR-X系统对单个珠子进行计数的能力提供了非常高的灵敏度,可以检测到极低浓度的蛋白质,研究人员可以在一次检测中最多检测六种不同的靶蛋白。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><strong>6. HiBit蛋白质检测系统</strong></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box" center="center"><p><img src="image/20201014/88b89a349e0fafaa0f8bbc4874b25696_10.jpg" /></p></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">由Promega公司生产的新型蛋白质检测系统擅长测量细胞内的蛋白质水平。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">”HiBiT标记系统旨在提供一个非常简单,灵敏的生物发光方法来测量细胞内部或者细胞表面的蛋白质。“高级研究科学家Chris Eggers说。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">当小而易于整合的11-氨基酸标签(高BiT或HiBiT)与互补的大BiT(LgBiT)156-氨基酸组分相互作用时,它们紧密结合并释放可检测的光,研究人员可以使用Promega的HiBit蛋白质检测系统,其可将任何位置的小Bit标签整合到蛋白质中。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">Promega还提供了免费授权HiBiT标签序列的选项,检测试剂起价为160美元,根据所需的试剂和体积,成本上升到8925美元。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">爱荷华大学的生物学家Julien Sebag一直在使用HiBit蛋白质检测系统研究G蛋白偶联受体(GPCR),他表示,HiBit蛋白质检测系统的速度令人感到非常满意,特别是与ELISA相比。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><strong>7.全基因组CRISPR筛选平台:Edit-R</strong></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box" center="center"><p><img src="image/20201014/7c235d8dd14e43199728f279e2ebdf67_11.jpg" /></p></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">Edit-R筛选平台于今年6月由Dharmacon公司推出,该平台为用户提供了一个排列合成crRNA指南的库,其中包含一个”每孔一个基因“的格式,以此可以进行更细微的检测。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">Dharmacon公司产品经理Baskin表示:”你可以测量一个表型的多种变异,以获得更复杂和更丰富的数据集。“</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">Edit-R还为每个基因提供了四种不同的指导RNA,由此,获得了大量客户,Baskin指出,“每个基因拥有多个数据点确实提高了统计效力。”</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">加利福尼亚大学旧金山分校的Judd Hultquist最近使用Edit-R作为研究HIV-宿主相互作用项目的一部分,Hultquist在给Dharmacon公司的电子邮件中写道:“易用性,高效率,广泛的可访问性和功能适应性使这个平台真正具有革命性。”</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><strong>8.Chromium控制器</strong></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box" center="center"><p><img src="image/20201014/ec7cef1b79003a5a03b2404235b52a3c_12.jpg" /></p></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">Chromium控制器可以使转录组和全基因组分析比以往更精确,今年10月,10XGenomics公司推出了Chromium Single Cell V(D)J Solution,分析T细胞和B细胞的适应性免疫受体和抗体库,并测量同一单细胞样品的基因表达。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">研究人员可将样品作为单细胞(<span>或长DNA分子</span>),连同试剂和单独条形码凝胶珠分成单个油滴,试剂裂解细胞,并与条形码珠一起,创建其RNA转录物的cDNA文库,然后对其进行测序,条形码是特定于每个液滴的,在Chromium软件处理数据之后,用户可以追踪单个细胞中的基因表达,10x基因组公司的战略营销负责人Mike Lucero表示:”数十万个液滴室的每个基因都是数字化的。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">约翰霍普金斯大学的计算生物学家迈克尔·沙茨表示:这项技术独一无二的特点是能够区分给定的等位基因是来自母系还是父系染色体,它就像一个新式的显微镜让我们看到了以前从未见过的东西。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><strong>9.TSQ Altis三阶段质谱仪</strong></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box" center="center"><p><img src="image/20201014/a7f777dc13a711cc0979c83e685c5359_13.jpg" /></p></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">赛默飞的TSQ Altis三阶段质谱仪能够稳定可靠地对大多数分析物类型进行定量分析,即使在复杂样品(<span>如血浆和组织</span>)中也如此,该系统可广泛用于法医毒理学和临床研究。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">Altis拥有三重四极杆,使研究人员可以针对特定的分子,并提供增强的离子传输一致性,该系统的另一个优势是主动式碰撞池,即电离样品与中性气体和碎片碰撞,确保快速,选择性的反应监测,从而提高生产率。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">纽约布法罗大学从事抗体药物开发和分析工作的屈军在5月份对Altis进行了测试之后,订购了一台TSQ Altis三阶段质谱仪,他表示,在测试中,TSQ Altis三阶段质谱仪能够检测出含有数十万个肽的样品中不必要的部分,这对于蛋白质分析是特别重要的。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><strong>10. Invitrogen TrueCut Cas9蛋白</strong></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box" center="center"><p><img src="image/20201014/f0d5a44ddcff3018ea3cdc6407c3e864_14.jpg" /></p></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">这种蛋白经过专门设计,可最大限度地提高Cas9蛋白的切割效率,从而加速生产过程,Cas9蛋白的切割效率是CRISPR-Cas9基因组编辑中的制约性步骤。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">TrueCut蛋白不仅可以在标准细胞系中进行高效编辑,还可以在干细胞和原代细胞中进行高效编辑。Fred Hutchinson癌症研究中心的科学家Olivier Humbert就使用TrueCut系统编辑血液干细胞,目的是开发β地中海贫血等血红蛋白病的治疗方法。</p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"><br /></p><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">目前,赛默飞提供两种浓度的TrueCut Cas9蛋白:标准编辑测定法为1微克,更具挑战性的测定法为5微克。</p><p><br /></p><p><span>来源:医谷(微信号:yigoonet) 作者: 史士云</span></p><section data-id="1658"><section><section><p><span><strong>相关阅读</strong></span></p></section><p><p><img src="image/20201014/ec237188ae1e9c03eb4d9814f31b18ab_15.gif" /></p></p><p><span>被进口“独霸”的DSA市场——国内外主流血管造影品牌大盘点</span></p><p><br /></p><p><span>『大开眼界』世界上第一个MRI兼容的监护仪获得FDA批准</span></p><p><br /></p><p><span>『大开眼界』中国又放出一波“高科技”</span></p><p><br /></p><p><span>“黑科技”集体亮相乌镇,未来将被这样颠覆!</span></p><p><br /></p><p><span>『大开眼界』全球首款六轴3D打印机问世——打印人体器官还远吗?</span></p><p><br /></p><p><span>麻省理工惊天发明:真实的远程3D交互技术,这科技太黑了!</span></p><p><br /></p><p><span>『大开眼界』国产首款一体式 PET/MR正式问世!</span></p><p><br /></p><p><span>『大开眼界』全球首例机器人视网膜手术 比人工操作靠谱</span></p></section></section><p><br /></p><p><p><img src="image/20201014/ddc723b50bcaa3c373cb35a0033daacd_16.jpg" /></p></p>
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