盘点2017年度值得关注的十大医疗技术发明

admin · 发表于 2020-10-14 17:29:57

<blockquote>让天下没有难找的医疗器械服务信息！“医疗器械助手” 让一切触手可及！<img src="image/20201014/ac7c4c86bff3f0660e634292dc511095_1.jpg" />点击图片<img src="image/20201014/c407fe90207c2622cc8e1dff32d28341_2.jpg" /><img src="image/20201014/c407fe90207c2622cc8e1dff32d28341_2.jpg" /><img src="image/20201014/c407fe90207c2622cc8e1dff32d28341_2.jpg" /> </blockquote> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">日前，科学家杂志（The Scientist）评选出了2017年度的十大医疗技术发明。 <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">1. IsoCode芯片<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box" center="center"><img src="image/20201014/8e4caa3416db521ceda28119d1ce89d1_5.jpg" /><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">这是一种新的单细胞技术，其能让研究人员能够根据细胞分泌的蛋白质对细胞进行特征分析，可以同时分析多达42种不同的细胞因子，趋化因子和其他分子。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">IsoCode芯片由Isoplexis公司于今年2月份推出，它包含数千个只能容纳单细胞的微型腔室，在每个微室内，15个分离空间的槽内包含多达三种针对特定分泌蛋白的不同抗体，结合后，抗体发出三种颜色的荧光，使研究人员能够区分蛋白质。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">目前，IsoCode芯片的潜在应用之一是分析CAR-T细胞，IsoPlexis公司首席执行官Sean Mackay表示：“IsoCode芯片能够同时分析数千个单独的T细胞或免疫细胞，更重要的是，每个免疫细胞能够获得30到45个分泌蛋白，而以往的技术要么大量地测量细胞，要么丢失颗粒度，要么只能看到每个细胞的少量分泌蛋白质，因此，IsoCode芯片是真正的创新”。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">科学家杂志的评审专家也评论到：“IsoCode单细胞技术，易于使用，有可能影响癌症研究的生物标志物发现和患者监测。”<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">2.雅培手持血液仪i-STAT Alinity<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box" center="center"><img src="image/20201014/966ef97d1be2313e85850f798968866f_6.jpg" /><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">雅培公司最新版本的手持式血液检测设备i-STAT Alinity具备了很多创新的功能，它的体积只有80年代的手机大小（很适合医护人员随身携带），却拥有三十年前难以想象的技术，i-STAT Alinity可以对几滴血样进行无数次测试，并在几分钟内将包括葡萄糖水平和血细胞比容的结果传送给医生，使得即时检测变得更加快速、便捷。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">雅培护理诊断业务研发总监纳Narendra Soman表示：“i-STAT的一项改进是大型彩色触摸屏，同时，如果患者的指标超出正常范围，则会向用户发出声音和视觉提示，此外，我们为测试结果还增加了更多功能，一旦获得血液检测结果，就可以从仪器传输到病人的病历”。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">一年前，i-STAT Alinity正式上市，目前已在大约四十多个国家有售（不包括美国），售价在7000到12500美元之间。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">3. 实验室培育类固醇激素：QGel<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box" center="center"><img src="image/20201014/a15227e06de1b7d49ec817f878e507ff_7.jpg" /><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">这个产品利用动物源性细胞外基质（ECM）技术，从患者的癌细胞种植类固醇，并用于制作个性化治疗，目前已在抗肿瘤研究中得以使用，比如，诺华就利用QGel来培育用于药物筛选用的癌细胞，和来自患者自身的细胞。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">QGel公司的联合创始人Colin Sanctuary表示，如果使用QGel而不是动物来源的ECMs来培育，那么在临床治疗中的应用将能够达到全球范围的规模，并对受影响的人群将产生重大影响。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">QGel的成本大约在4,000美元至5,000美元之间。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">4.Blaze系统<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box" center="center"><img src="image/20201014/e32a56e7ef6bb479f87d00084916d26b_8.jpg" /><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">Intabio公司的Blaze系统用于检测和鉴定蛋白质异构体，可以为制药公司在实验室的准备工作中节省大量的时间，”通常需要一个月的蛋白质分析，Blaze一天内就能完成。“Intabio公司首席执行官Lena吴表示。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">Blaze系统将检测、定量、鉴定整合到一个微流体系统中，该系统能够在检测后立即发送蛋白质用于质谱分析，避免了单独为质谱分析准备材料的繁琐过程，以此加快了检测的速度，”它彻底改变了能获得产品质量关键信息的途径。“Lena吴说道。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">Blaze系统将在几个月后正式上市，定价尚未确定，不过有专家预估该设备价格在7万美元至20万美元之间。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">5、SR-X超敏感生物标志物检测系统<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box" center="center"><img src="image/20201014/4e0d0a428fa77178f7bb01cf8d0b2708_9.jpg" /><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">今年8月，Quanterix公司正式推出SR-X系统，该生物标志物检测系统可以提供超过80种不同的分析方法来测试样品，通常是血液或血清，不过，也能通过分析脑脊液或单细胞裂解液来检测细胞因子，神经变性或神经炎症等其他标志物。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">Quanterix公司产品战略总监Jeremy Lambert表示：SR-X的核心技术Simoa使用更多的磁珠相作用于它们的目标蛋白质，每个珠只捕获一个蛋白质，然后将那些携有蛋白质的颗粒沉淀、洗涤，再与抗体检测器结合，并流过可容纳单一颗粒的200,000个微室的阵列，在那里，抗体检测器与荧光报道分子相互作用，SR-X系统对单个珠子进行计数的能力提供了非常高的灵敏度，可以检测到极低浓度的蛋白质，研究人员可以在一次检测中最多检测六种不同的靶蛋白。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">6. HiBit蛋白质检测系统<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box" center="center"><img src="image/20201014/88b89a349e0fafaa0f8bbc4874b25696_10.jpg" /><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">由Promega公司生产的新型蛋白质检测系统擅长测量细胞内的蛋白质水平。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">”HiBiT标记系统旨在提供一个非常简单，灵敏的生物发光方法来测量细胞内部或者细胞表面的蛋白质。“高级研究科学家Chris Eggers说。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">当小而易于整合的11-氨基酸标签（高BiT或HiBiT）与互补的大BiT（LgBiT）156-氨基酸组分相互作用时，它们紧密结合并释放可检测的光，研究人员可以使用Promega的HiBit蛋白质检测系统，其可将任何位置的小Bit标签整合到蛋白质中。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">Promega还提供了免费授权HiBiT标签序列的选项，检测试剂起价为160美元，根据所需的试剂和体积，成本上升到8925美元。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">爱荷华大学的生物学家Julien Sebag一直在使用HiBit蛋白质检测系统研究G蛋白偶联受体（GPCR），他表示，HiBit蛋白质检测系统的速度令人感到非常满意，特别是与ELISA相比。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">7.全基因组CRISPR筛选平台：Edit-R<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box" center="center"><img src="image/20201014/7c235d8dd14e43199728f279e2ebdf67_11.jpg" /><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">Edit-R筛选平台于今年6月由Dharmacon公司推出，该平台为用户提供了一个排列合成crRNA指南的库，其中包含一个”每孔一个基因“的格式，以此可以进行更细微的检测。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">Dharmacon公司产品经理Baskin表示：”你可以测量一个表型的多种变异，以获得更复杂和更丰富的数据集。“<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">Edit-R还为每个基因提供了四种不同的指导RNA，由此，获得了大量客户，Baskin指出，“每个基因拥有多个数据点确实提高了统计效力。”<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">加利福尼亚大学旧金山分校的Judd Hultquist最近使用Edit-R作为研究HIV-宿主相互作用项目的一部分，Hultquist在给Dharmacon公司的电子邮件中写道：“易用性，高效率，广泛的可访问性和功能适应性使这个平台真正具有革命性。”<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">8.Chromium控制器<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box" center="center"><img src="image/20201014/ec7cef1b79003a5a03b2404235b52a3c_12.jpg" /><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">Chromium控制器可以使转录组和全基因组分析比以往更精确，今年10月，10XGenomics公司推出了Chromium Single Cell V（D）J Solution，分析T细胞和B细胞的适应性免疫受体和抗体库，并测量同一单细胞样品的基因表达。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">研究人员可将样品作为单细胞（或长DNA分子），连同试剂和单独条形码凝胶珠分成单个油滴，试剂裂解细胞，并与条形码珠一起，创建其RNA转录物的cDNA文库，然后对其进行测序，条形码是特定于每个液滴的，在Chromium软件处理数据之后，用户可以追踪单个细胞中的基因表达，10x基因组公司的战略营销负责人Mike Lucero表示：”数十万个液滴室的每个基因都是数字化的。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">约翰霍普金斯大学的计算生物学家迈克尔·沙茨表示：这项技术独一无二的特点是能够区分给定的等位基因是来自母系还是父系染色体，它就像一个新式的显微镜让我们看到了以前从未见过的东西。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">9.TSQ Altis三阶段质谱仪<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box" center="center"><img src="image/20201014/a7f777dc13a711cc0979c83e685c5359_13.jpg" /><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">赛默飞的TSQ Altis三阶段质谱仪能够稳定可靠地对大多数分析物类型进行定量分析，即使在复杂样品（如血浆和组织）中也如此，该系统可广泛用于法医毒理学和临床研究。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">Altis拥有三重四极杆，使研究人员可以针对特定的分子，并提供增强的离子传输一致性，该系统的另一个优势是主动式碰撞池，即电离样品与中性气体和碎片碰撞，确保快速，选择性的反应监测，从而提高生产率。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">纽约布法罗大学从事抗体药物开发和分析工作的屈军在5月份对Altis进行了测试之后，订购了一台TSQ Altis三阶段质谱仪，他表示，在测试中，TSQ Altis三阶段质谱仪能够检测出含有数十万个肽的样品中不必要的部分，这对于蛋白质分析是特别重要的。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">10. Invitrogen TrueCut Cas9蛋白<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box" center="center"><img src="image/20201014/f0d5a44ddcff3018ea3cdc6407c3e864_14.jpg" /><p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">这种蛋白经过专门设计，可最大限度地提高Cas9蛋白的切割效率，从而加速生产过程，Cas9蛋白的切割效率是CRISPR-Cas9基因组编辑中的制约性步骤。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">TrueCut蛋白不仅可以在标准细胞系中进行高效编辑，还可以在干细胞和原代细胞中进行高效编辑。Fred Hutchinson癌症研究中心的科学家Olivier Humbert就使用TrueCut系统编辑血液干细胞，目的是开发β地中海贫血等血红蛋白病的治疗方法。<p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box"> <p yahei="yahei" important="important" none="none" normal="normal" rgb="rgb" px="px" microsoft="microsoft" neue="neue" helvetica="helvetica" hiragino="hiragino" sans="sans" gb="gb" arial="arial" sans-serif="sans-serif" both="both" break-word="break-word" em="em" border-box="border-box">目前，赛默飞提供两种浓度的TrueCut Cas9蛋白：标准编辑测定法为1微克，更具挑战性的测定法为5微克。 来源：医谷（微信号：yigoonet）作者：史士云<section data-id="1658"><section><section>相关阅读</section><img src="image/20201014/ec237188ae1e9c03eb4d9814f31b18ab_15.gif" />被进口“独霸”的DSA市场——国内外主流血管造影品牌大盘点 『大开眼界』世界上第一个MRI兼容的监护仪获得FDA批准 『大开眼界』中国又放出一波“高科技” “黑科技”集体亮相乌镇，未来将被这样颠覆！ 『大开眼界』全球首款六轴3D打印机问世——打印人体器官还远吗？ 麻省理工惊天发明：真实的远程3D交互技术，这科技太黑了！ 『大开眼界』国产首款一体式 PET/MR正式问世！ 『大开眼界』全球首例机器人视网膜手术比人工操作靠谱</section></section> <img src="image/20201014/ddc723b50bcaa3c373cb35a0033daacd_16.jpg" />

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