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<section data-role="paragraph" data-color="rgb(182, 228, 253)" data-custom="rgb(182, 228, 253)"><section><section><section><section powered-by="gulangu"><section><section><section><section powered-by="gulangu"><section><section><section><section powered-by="gulangu"><section><section><section><section powered-by="gulangu"><section><section><section><section><p><p><img src="image/20201014/86b79d371e9862b01189f0252a03b2cd_1.png" /></p></p></section><section><section><span><strong></strong></span></section><p><span>医疗器械媒体报道先锋</span></p><p><span>分享专业医疗器械知识</span></p></section><section><section><section><span>关注</span></section></section></section></section></section></section></section></section></section></section></section></section></section></section></section></section></section></section></section></section></section></section></section><p><span>2月14日，CRISPR基因编辑的大牛张锋教授团队发布了使用基于CRISPR/Cas13的SHERLOCK技术检测新型冠状病毒的路径。测试表明，SHERLOCK技术检测新冠病毒异常灵敏，每微升里仅10-100个病毒拷贝，就可以被检测出来。而且，该方法操作简单，一个小时内即可完成检测。</span></p><p><span><br /></span></p><p><p><img src="image/20201014/ed71549268b7ac686346d283d56fbd4b_2.png" /></p></p><p><br /></p><p><span>SHERLOCK，由张锋开发并命名，同时，SHERLOCK是神探夏洛克·福尔摩斯（Sherlock Holmes）的名字。SHERLOCK技术由张锋团队于2017年发明，此后在拉沙热、埃博拉、寨卡病毒、登革热等RNA病毒疫情检测中大显身手。随后，SHERLOCKv2 诞生了，与 SHERLOCK 相比，其灵敏度提高了100倍，并能在同一样品中检测出多种病毒感染，比如能同时检出寨卡和登革热病毒。</span></p><p><span><br /></span></p><p><p><img src="image/20201014/d76e350a52fc69aaa34f116719f66cae_3.jpg" /></p></p><p><span></span><br /></p><p><span>之所以能达到这样效果，是因为他们在 SHERLOCKv2 中引入了多种来自不同种属细菌的 Cas13 酶，如 LwaCas13a 和 PsmCas13b。Cas13是一种依赖于RNA靶向的RNA核酸酶。世界上许多最常见或致命的人类病原体都是RNA病毒（例如埃博拉病毒、艾滋病病毒，流感病毒、SARS病毒等）此次在武汉爆发的新型冠状病毒，同样是RNA病毒。</span></p><p><span><br /></span></p><p><span>针对此次新型冠状病毒，研究设计了两个向导RNA（gRNA）,分别用于识别新冠病毒的S基因和Orf1ab基因。一旦检测样本中含有新型冠状病毒，向导RNA就会识别到它的S基因和Orf1ab基因，然后形成视觉可见的结果。</span></p><p><span><br /></span></p><p><p><img src="image/20201014/e7940a1f2031e34aeab24ab5c8e3d429_4.png" /></p></p><p><br /></p><p><span>但是 张锋团队指出由于他们无法获取新型冠状病毒患者样本，因此无法使用真实的患者样本来验证这一技术。同时，他们欢迎和有新型冠状病毒肺炎（COVID-19）样本的研究人员合作，并且可以免费提供用于检测的试剂盒。</span></p><p><span><br /></span></p><p><span>张锋团队研发用于快速诊断感染的便宜设备，有望为全球，特别是发展中国家的病毒比如：新冠，塞卡感染诊断，带来一场真正的革命。</span></p><p><br /></p><p><span>整理收集自中国生物技术网，DeepTech深科技，Bioworld</span></p><section data-role="outer" label="Powered by gulangu"><section data-role="paragraph" data-color="#27939d"><p><a 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发表于 2020-10-14 12:56:10