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全自动血液细胞分析仪血红蛋白浓度和红细胞/血小板测量原理

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发表于 2020-10-19 13:26:03 | 显示全部楼层 |阅读模式

                    

                    

                    
                    
                    <section><section powered-by="gulangu"><section><section><p><img src="image/20201019/80ca87eafc34d0d6e75a626e84d383c5_1.gif" /></p></section></section></section><section powered-by="gulangu"><section><section><p><br  /></p></section></section></section><section powered-by="gulangu"><section><section><section><section powered-by="gulangu"><section><section><p>血红蛋白浓度测量原理</p></section></section></section></section></section></section></section><section powered-by="gulangu"><section><section><p>&nbsp; &nbsp; &nbsp; &nbsp;全自动血红蛋白浓度测量一般采用比色法。根据朗伯-比尔定律,一束平行的单色光垂直通过某一均匀的、非散射的吸光物质溶液时,其吸光度A与溶液层厚度L和浓度C的乘积成正比,经过试剂处理后的HGB作为吸光物质,根据定律可推导出HGB的浓度与吸光度的关系,从而达到通过测量吸光度达到测量HGB浓度目的。</p></section></section></section><section powered-by="gulangu"><section><section><p><img src="image/20201019/8fe7b997cd168bc5e1ec9f1842d0516c_2.jpg" /></p></section></section></section><section powered-by="gulangu"><section><section><p>&nbsp; &nbsp; &nbsp; &nbsp;血红蛋白浓度(HGB)由以下公式计算得到,单位为g/L。</p><p>&nbsp; &nbsp; &nbsp; &nbsp;HGB=常数*Ln(本底透过光强/样本透过光强)</p></section></section></section><section powered-by="gulangu"><section><section><p><br  /></p></section></section></section><section powered-by="gulangu"><section><section><section><section powered-by="gulangu"><section><section><p>红细胞/血小板测量原理</p></section></section></section></section></section></section></section><section powered-by="gulangu"><section><section><p>&nbsp; &nbsp; &nbsp; &nbsp;设计传感器使RBC、PLT在流体“聚焦”的作用下,排队通过宝石小孔,根据库尔特原理产生相应的脉冲,后端处理装置将采集到的电脉冲放大后与正常的红细胞/血小板体积范围所对应的通道电压阀值相比较,计算出电脉冲幅度落在红细胞/血小板通道内的电脉冲个数。由此,所有采集到的电脉冲根据不同的通道电压阀值进行了分类,落在红细胞/血小板通道内的电脉冲个数就是红细胞/血小板的个数。依据脉冲电压幅度划分的每一个通道范围内的细胞个数决定了细胞的体积分布。用横坐标表示细胞体积,纵坐标表示细胞相对数量的二维图就是反映细胞群体分布情况的直方图。</p></section></section></section><section powered-by="gulangu"><section><section><p><img src="image/20201019/e874ea4030901abb958daf2ce1fd1acb_3.jpg" /></p></section></section></section><section powered-by="gulangu"><section><section><p><br  /></p></section></section></section><section powered-by="gulangu"><section><section><section powered-by="gulangu"><section><section><p>欢</p></section></section></section></section><section><section powered-by="gulangu"><section><section><p>迎</p></section></section></section></section><section><section powered-by="gulangu"><section><section><p>关</p></section></section></section></section><section><section powered-by="gulangu"><section><section><p>注</p></section></section></section></section></section></section><section powered-by="gulangu"><section><section><p><br  /></p></section></section></section><section powered-by="gulangu"><section><section><section powered-by="gulangu"><section><section><p><img src="image/20201019/868d7891b99956b469014b3b51719c0c_4.jpg" /></p></section></section></section></section><section><section powered-by="gulangu"><section><section></section></section></section></section><section><section powered-by="gulangu"><section><section><p>关注我们</p><p>一起涨姿势!</p></section></section></section></section></section></section></section>
               
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